中英文说明书丨MyBioSource 犬尿氨酸 ELISA试剂盒

　　MBS495082 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kynurenine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Kynurenine. The ELISA analytical biochemical technique of the MBS495082 kit is based on Kynurenine antibody-Kynurenine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Kynurenine antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Kynurenine. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

　　Intended Uses:

　　Enzyme Immunoassay for the quantitative determination of L-Kynurenine in serum, plasma and various biological samples. After acylation Kynurenine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction Is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.

　　Principle of the Assay:

　　Enzyme Immunoassay for the quantitative determination of L-Kynurenine in serum, plasma and various biological samples. After acylation Kynurenine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction Is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.

　　艾美捷 MyBioSource犬尿氨酸 ELISA试剂盒是一种即用型微孔板或全板 ELISA（酶联免疫吸附测定）试剂盒，用于分析生物样品中犬尿氨酸、ELISA 试剂盒目标分析物的存在。试剂盒标准品或阳性对照的浓度梯度为含有犬尿氨酸的生物研究样品提供了理论上的试剂盒检测范围。

　　艾美捷 MyBioSource犬尿氨酸 ELISA试剂盒测定原理：

　　酶免疫测定法用于定量测定血清、血浆和各种生物样品中的 L-犬尿氨酸。酰化后犬尿氨酸通过ELISA定量测定。竞争性 ELISA 使用微量滴定板格式。抗原与微量滴定板的固相结合。酰化的标准品、对照品和样品与固相结合的分析物竞争固定数量的抗体结合位点。当系统处于平衡状态时，通过洗涤除去游离抗原和游离抗原-抗体复合物。通过使用 TMB 作为底物的抗兔 IgG-过氧化物酶偶联物检测与固相结合的抗体。在 450 nm 监测反应。未知样品的定量是通过将它们的吸光度与用已知标准制备的参考曲线进行比较来实现的。

　　艾美捷 MyBioSource犬尿氨酸 ELISA试剂盒典型检测数据/标准曲线（仅供参考）：

　　

　　相关参考文献：

　　(1) Barone, P. (2019). The 'Yin' and the 'Yang' of the kynurenine pathway: excitotoxicity and neuroprotection

　　imbalance in stress-induced disorders. Behav Pharmacol, 30(2 and 3-Spec Issue), 163-186.

　　doi:10.1097/fbp.0000000000000477

　　(2) Bonaccorso, S., Marino, V., Puzella, A., Pasquini, M., Biondi, M., Artini, M., . . . Maes, M. (2002).

　　Increased depressive ratings in patients with hepatitis C receiving interferon-alpha-based immunotherapy

　　are related to interferon-alpha-induced changes in the serotonergic system. J Clin Psychopharmacol,

　　22(1), 86-90. doi:10.1097/00004714-200202000-00014

　　(3) Bryleva, E. Y., & Brundin, L. (2017). Kynurenine pathway metabolites and suicidality.

　　Neuropharmacology, 112(Pt B), 324-330. doi:10.1016/j.neuropharm.2016.01.034

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